Quantification & Protein Determination
Purpose : Bradford method를 통해 단백질을 quantification하고 determination 해준다.
Principle:
• Bradfrod method:
M.bradford가 개발한 단백질 정량법. 단백질에 결합하는 색소인 쿠마시브릴리언트(Coomassie Brilliant)블루 G250이 단백질에 결합하면 그 극대흡수가 변화하는 것을 이용한
damaged
C: global brand may be seriously damaged, leading to loss of brand equity
Ipanema-Introduction
The most cosmopolitan and wealthy neighborhood.
Legendary beach, restaurants, discos, nightclubs and luxurious apartments.
Chic boutiques, art galleries, theatres and cinemas.
Measuring Impact on Revenue
Direct Factors
Duration
% of local population
Consistency among
prot
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(1) Sample Preparation
Principle:
• Extraction Buffer(=lysis buffer) : Tris, TritonX-100, NP-40, SDS 등 여러 가지 detergent들이 cell wall과 mitochondria membrane을 깨트려주고 아래의 표에 제시된 protease inhibitors들이 세포 안의 protease가 단백질을 분해하는 것을 막아준다. 추가적으로 phosphatase inhibitor를 넣어 우
Stem cell calssification
1. Based upon location
Embryonic stem cells
Somatic/ adult stem cells
2. Based upon function
Totipotent
Pluripotent
Multipotent
Unipotent
Reject Response
Traditional Immuno-repressents
Constant administration
Little engraftment after 3~4 weeks
Host immune system impaired
Vulnerable to Infection
↓Need brief immuno-re
Late 1997 after the financial crisis due to interlayer polarization in the labor market share decline, the difficult living and low incomes continue to increase. since 2007 the global financial crisis, rising unemployment, income polarization, deterioration of family income, inflation, Household debt and interest burden due to increased sales of credit by various factors, including difficulties i